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Addgene

pCAGGS_VP35_MARV
(Plasmid #135471)

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Item Catalog # Description Quantity Price (USD)
Plasmid 135471 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VP35
  • Species
    Marburg marburgvirus, Musoke variant
  • Mutation
    None
  • Promoter AG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI/StuI (not destroyed)
  • 3′ cloning site EcoRI/SmaI (destroyed during cloning)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

VP35 gene flanked by BamHI sites

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS_VP35_MARV was a gift from Elke Mühlberger (Addgene plasmid # 135471 ; http://n2t.net/addgene:135471 ; RRID:Addgene_135471)

  • For your References section:

    Recombinant Marburg virus expressing EGFP allows rapid screening of virus growth and real-time visualization of virus spread. Schmidt KM, Schumann M, Olejnik J, Krahling V, Muhlberger E. J Infect Dis. 2011 Nov;204 Suppl 3:S861-70. doi: 10.1093/infdis/jir308. 10.1093/infdis/jir308 PubMed 21987762
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