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TDH3-STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-tCYC
(Plasmid #135465)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135465 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRG205
  • Backbone manufacturer
    Robert Gnuegge
  • Backbone size w/o insert (bp) 5660
  • Total vector size (bp) 9565
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)-LexAVP16
  • Species
    Synthetic
  • Insert Size (bp)
    3915
  • Promoter TDH3
  • Tag / Fusion Protein
    • BFP, flag

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGTAGGTATTGATTGTAATTCTGTAAATC
  • 3′ sequencing primer CTTCAGGTTGTCTAACTCCTTCCTTTTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For integration in yeast, linearize plasmid with AscI.

The reporter genes associated with this construct are #58432, #58433 and #58434 published in: Nucleic Acids Res. 42, e130–e130 (2014)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TDH3-STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-tCYC was a gift from Alice Ting (Addgene plasmid # 135465 ; http://n2t.net/addgene:135465 ; RRID:Addgene_135465)

  • For your References section:

    Directed evolution improves the catalytic efficiency of TEV protease. Sanchez MI, Ting AY. Nat Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 10.1038/s41592-019-0665-7. 10.1038/s41592-019-0665-7 PubMed 31819267
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