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Addgene

CMV:eGFP-CaM-uTEV1Δ(220-242)
(Plasmid #135461)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135461 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3802
  • Total vector size (bp) 1944
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP-CaM-uTEV1Δ
  • Species
    Synthetic
  • Mutation
    S219V mutation improves stability and S153N improves catalytic efficency
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP, V5

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ggcaccaaaatcaacgggactttcc
  • 3′ sequencing primer CAGTGGGAGTGGCACCTTCCAGGGTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV:eGFP-CaM-uTEV1Δ(220-242) was a gift from Alice Ting (Addgene plasmid # 135461 ; http://n2t.net/addgene:135461 ; RRID:Addgene_135461)

  • For your References section:

    Directed evolution improves the catalytic efficiency of TEV protease. Sanchez MI, Ting AY. Nat Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 10.1038/s41592-019-0665-7. 10.1038/s41592-019-0665-7 PubMed 31819267
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