CMV:eGFP-p2A-HA-β2Ar-uTEV1Δ(220-242)
(Plasmid
#135459)
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PurposeMammalian expression of SPARK protease component
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 135459 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLX208
- Backbone size w/o insert (bp) 6775
- Total vector size (bp) 9568
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameeGFP-p2A-HA-βarrestin2-uTEV1Δ
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SpeciesSynthetic
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Insert Size (bp)2793
- Promoter CMV
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Tag
/ Fusion Protein
- eGFP, HA
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-f
- 3′ sequencing primer WPRE-r (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Hygromycin selection gene was deleted
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV:eGFP-p2A-HA-β2Ar-uTEV1Δ(220-242) was a gift from Alice Ting (Addgene plasmid # 135459 ; http://n2t.net/addgene:135459 ; RRID:Addgene_135459) -
For your References section:
Directed evolution improves the catalytic efficiency of TEV protease. Sanchez MI, Ting AY. Nat Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 10.1038/s41592-019-0665-7. 10.1038/s41592-019-0665-7 PubMed 31819267
