Gal:mCherry-uTEV3
(Plasmid
#135453)
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PurposeYeast expression of uTEV3 fused to mCherry
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 135453 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRSII 413
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Backbone manufacturerSteven B. Haase
- Backbone size w/o insert (bp) 5807
- Total vector size (bp) 7361
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsGalactose media to induce expression
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemCherry-uTEv3
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SpeciesSynthetic
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Insert Size (bp)1566
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MutationS219V mutation improves stability , I138T/S153N/T180A mutations improve catalytic activity
- Promoter Gal
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Tag
/ Fusion Protein
- mCherry
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Gal-f
- 3′ sequencing primer tCYC-r (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Gal:mCherry-uTEV3 was a gift from Alice Ting (Addgene plasmid # 135453 ; http://n2t.net/addgene:135453 ; RRID:Addgene_135453) -
For your References section:
Directed evolution improves the catalytic efficiency of TEV protease. Sanchez MI, Ting AY. Nat Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 10.1038/s41592-019-0665-7. 10.1038/s41592-019-0665-7 PubMed 31819267
