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pcDNA5-Lifeact_Halo_T2A_EGFP
(Plasmid #135445)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135445 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    thermofisher
  • Backbone size w/o insert (bp) 5137
  • Total vector size (bp) 6877
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lifact-HaloTag7 and EGFP
  • Insert Size (bp)
    1739
  • Promoter CMV/TetO
  • Tag / Fusion Protein
    • Lifact (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-Lifeact_Halo_T2A_EGFP was a gift from Kai Johnsson (Addgene plasmid # 135445 ; http://n2t.net/addgene:135445 ; RRID:Addgene_135445)

  • For your References section:

    Photoactivation of silicon rhodamines via a light-induced protonation. Frei MS, Hoess P, Lampe M, Nijmeijer B, Kueblbeck M, Ellenberg J, Wadepohl H, Ries J, Pitsch S, Reymond L, Johnsson K. Nat Commun. 2019 Oct 8;10(1):4580. doi: 10.1038/s41467-019-12480-3. 10.1038/s41467-019-12480-3 PubMed 31594948
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