pcDNA5-TOM20_Halo_T2A_EGFP
(Plasmid
#135443)
-
PurposeMamalian cell expression of mictochondrial HaloTag7 and cytosolic EGFP
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 135443 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepcDNA5/FRT/TO
-
Backbone manufacturerthermofisher
- Backbone size w/o insert (bp) 5137
- Total vector size (bp) 7261
-
Vector typeMammalian Expression
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameTOM20-HaloTag7 and EGFP
-
Insert Size (bp)2122
- Promoter CMV/TetO
-
Tag
/ Fusion Protein
- TOM20 (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV_Fwd
- 3′ sequencing primer BGH_Rev (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA5-TOM20_Halo_T2A_EGFP was a gift from Kai Johnsson (Addgene plasmid # 135443 ; http://n2t.net/addgene:135443 ; RRID:Addgene_135443) -
For your References section:
Photoactivation of silicon rhodamines via a light-induced protonation. Frei MS, Hoess P, Lampe M, Nijmeijer B, Kueblbeck M, Ellenberg J, Wadepohl H, Ries J, Pitsch S, Reymond L, Johnsson K. Nat Commun. 2019 Oct 8;10(1):4580. doi: 10.1038/s41467-019-12480-3. 10.1038/s41467-019-12480-3 PubMed 31594948
