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pEM03
(Plasmid #135417)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135417 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEM01
  • Backbone manufacturer
    Edgar Medina
  • Backbone size w/o insert (bp) 5885
  • Total vector size (bp) 6961
  • Modifications to backbone
    replaced CMVpr by digestion with SacI-HF & PacI and SLIC cloning Hsp70pr
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpunHsp70pr
  • Alt name
    Spizellomyces Hsp70pr
  • Species
    Spizellomyces punctatus
  • Insert Size (bp)
    1070
  • Promoter Hsp70pr

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CACTTTATGCTTCCGGCTCCTATGTT
  • 3′ sequencing primer CTGCCGGTAGAGGTGTGGTCAATA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEM03 was a gift from Nicolas Buchler (Addgene plasmid # 135417 ; http://n2t.net/addgene:135417 ; RRID:Addgene_135417)

  • For your References section:

    Genetic transformation of Spizellomyces punctatus, a resource for studying chytrid biology and evolutionary cell biology. Medina EM, Robinson KA, Bellingham-Johnstun K, Ianiri G, Laplante C, Fritz-Laylin LK, Buchler NE. Elife. 2020 May 11;9. pii: 52741. doi: 10.7554/eLife.52741. 10.7554/eLife.52741 PubMed 32392127
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