pcDNA3.1-hRARγ.hRXRα
(Plasmid #135416)

• Purpose: Human Retinoic Acid Receptor-gamma and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector
• Depositing Lab: Catharine Ross
• Publication: Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. doi: 10.1016/j.ab.2019.04.022. Epub 2019 Apr 27.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 8182
• Modifications to backbone: Neomycin gene was replaced with RXRα.
• Vector type: Mammalian Expression
• Selectable markers: None

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Follow the protocol from Promega.
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Human retinoic acid receptor-gamma
• Alt name: RARγ
• Species: H. sapiens (human)
• Insert Size (bp): 1365
• GenBank ID: NM_000966
• Promoter: CMV

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: ATGCTAGCGCCACCATGGCCACCAATAAGGAGC
• 3′ sequencing primer: TTAAGCTTCAGGCTGGGGACTTCAGGC

Gene/Insert 2

• Gene/Insert name: Human retinoid X receptor-alpha
• Alt name: RXRα
• Species: H. sapiens (human)
• Insert Size (bp): 1389
• GenBank ID: NM_002957
• Promoter: SV40

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: SmaI (destroyed during cloning)
• 3′ cloning site: BstBI (not destroyed)
• 5′ sequencing primer: GCCACCATGGACACCAAACATTTCCTG
• 3′ sequencing primer: TATTCGAACTAAGTCATTTGGTGCGGCG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

RARγ was cloned in the multiple cloning site at NheI/HindIII. Neomycin gene was removed and replaced with RXRα at the sites of SmaI/BstBI.