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Addgene

pCW57.1 Lysosomal-METRIQ
(Plasmid #135401)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135401 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMRX-IP
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DNAse II alpha
  • Alt name
    DNASE2
  • Alt name
    Deoxyribonuclease-2-alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1080
  • Mutation
    Single-nucleotide polymorphism C432T
  • GenBank ID
    NM_001375.2
  • Entrez Gene
    DNASE2 (a.k.a. DNASE2A, DNL, DNL2)
  • Tags / Fusion Proteins
    • sfGFP (C terminal on insert)
    • T2A (C terminal on insert)
    • mCherry

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW57.1 Lysosomal-METRIQ was a gift from Eisuke Itakura (Addgene plasmid # 135401 ; http://n2t.net/addgene:135401 ; RRID:Addgene_135401)

  • For your References section:

    Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probe. Ishii S, Matsuura A, Itakura E. Sci Rep. 2019 Aug 12;9(1):11635. doi: 10.1038/s41598-019-48131-2. 10.1038/s41598-019-48131-2 PubMed 31406169
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