pcDNA3.1-hRXRα
(Plasmid #135400)

• Purpose: Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using CMV promoter
• Depositing Lab: Catharine Ross
• Publication: Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. doi: 10.1016/j.ab.2019.04.022. Epub 2019 Apr 27.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6817
• Modifications to backbone: The human RXRα open reading frame PCR amplified DNA was first cloned into pGEMT-Easy vector (Promega) and then subjected to DNA sequencing for confirmation. The isolated plasmid containing the receptor DNA was digested with EcoRI and subcloned into the pcDNA3.1(+) expression vector.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Follow the protocol from Promega
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Human retinoid X receptor- alpha
• Alt name: RXRα
• Species: H. sapiens (human)
• Insert Size (bp): 1389
• GenBank ID: NM_002957
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GCCACCATGGACACCAAACATTTCCTG
• 3′ sequencing primer: TATTCGAACTAAGTCATTTGGTGCGGCG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The human RXRα open reading frame PCR amplified DNA was first cloned into pGEMT-Easy vector (Promega) and then subjected to DNA sequencing for confirmation. The isolated plasmid containing the receptor DNA was digested with EcoRI and subcloned into the pcDNA3.1(+) expression vector.