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PurposeCre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 135391 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 4891
- Total vector size (bp) 7073
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTeLC-P2A-EYFP
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Alt nameTeLC
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Alt nameEYFP
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SpeciesSynthetic
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Insert Size (bp)2182
- Promoter hSynapsin
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer Loxp-F
- 3′ sequencing primer Loxp-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Funded by the BRAIN initiative
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE was a gift from Fan Wang (Addgene plasmid # 135391 ; http://n2t.net/addgene:135391 ; RRID:Addgene_135391)