OSCA1.2-PP-GFP
(Plasmid #135390)

• Purpose: Expresses OSCA1.2 in mammalian cells
• Depositing Lab: Ardem Patapoutian
• Publication: Jojoa-Cruz et al Elife. 2018 Nov 1;7. pii: 41845. doi: 10.7554/eLife.41845.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 6156
• Total vector size (bp): 8475
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: OSCA1.2
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 2313
• GenBank ID: NM_118333.5
• Entrez Gene: AT4G22120 (a.k.a. AT4G22120, F1N20.220, F1N20_220)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP tag connected to the gene via a precision protease cleavable linker (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-Forward
• 3′ sequencing primer: BGHR-Reverse

Resource Information

• Supplemental Documents:
  • OSCA1.2-pp-EGFP.gb
• A portion of this plasmid was derived from a plasmid made by: Gene was human codon-optimized and synthesized by Genewiz. Precision protease cleavage linker and GFP was inserted by the lab.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry