pRSET FLIPE-100u
(Plasmid #13539)

• Depositing Lab: Wolf Frommer
• Publication: Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 102(24):8740-5.

Backbone

• Vector backbone: pRSET-B
• Backbone size w/o insert (bp): 2897
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FLIP-E S118W
• Alt name: ECFP-ybeJ-Venus
• Alt name: Glutamate/aspartate binding protein
• Alt name: gltI
• Species: E. coli
• Insert Size (bp): 2400
• Mutation: S118W in binding protein
• GenBank ID: NP_415188
• Tags / Fusion Proteins:
  • ECFP (N terminal on insert)
  • Venus (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Venus is from Dr. Atsushi Miyawaki. Please ask Dr. Miyawaki for permission to use Venus in another vector.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html