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Addgene

pTK234
(Plasmid #13535)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13535 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSET-B
  • Backbone size w/o insert (bp) 2897
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLIPW-CTTY
  • Alt name
    ECFP-TrpR-TrpR-Venus
  • Alt name
    TrpR
  • Species
    E. coli
  • Insert Size (bp)
    2200
  • Tags / Fusion Proteins
    • See map
    • See map

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Venus is from Dr. Atsushi Miyawaki.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html

1st TrpR was gateway cloned into FLIP cassette. 2nd TrpR is cloned into EcoRI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTK234 was a gift from Wolf Frommer (Addgene plasmid # 13535 ; http://n2t.net/addgene:13535 ; RRID:Addgene_13535)

  • For your References section:

    Nanosensor detection of an immunoregulatory tryptophan influx/kynurenine efflux cycle. Kaper T, Looger LL, Takanaga H, Platten M, Steinman L, Frommer WB. PLoS Biol. 2007 Oct;5(10):e257. 10.1371/journal.pbio.0050257 PubMed 17896864
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