Skip to main content
Addgene

pTK222
(Plasmid #13532)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13532 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLIPW-CTYT
  • Alt name
    ECFP-TrpR-Venus-TrpR
  • Alt name
    TrpR
  • Species
    E. coli
  • Insert Size (bp)
    2200
  • Tags / Fusion Proteins
    • See map
    • See map

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Venus is from Dr. Atsushi Miyawaki.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTK222 was a gift from Wolf Frommer (Addgene plasmid # 13532 ; http://n2t.net/addgene:13532 ; RRID:Addgene_13532)

  • For your References section:

    Nanosensor detection of an immunoregulatory tryptophan influx/kynurenine efflux cycle. Kaper T, Looger LL, Takanaga H, Platten M, Steinman L, Frommer WB. PLoS Biol. 2007 Oct;5(10):e257. 10.1371/journal.pbio.0050257 PubMed 17896864
Related items:
Commonly requested with: