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Addgene

flex-ChrimsonR-EYFP-Kv
(Plasmid #135319)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135319 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4827
  • Total vector size (bp) 6807
  • Modifications to backbone
    EF1a promoter exchanged for synapsin
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ChrimsonR
  • Species
    Synthetic
  • Insert Size (bp)
    1980
  • GenBank ID
    KF992060
  • Promoter synapsin
  • Tag / Fusion Protein
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer cttTATACGAAGTTatgCTAGcgccaCCatggctgagctgatca
  • 3′ sequencing primer ATTATACGAAGTTATGGCGCGCCttaaAACCGCGTAGCCTCTGGAA
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ChrimsonR sequence amplified from Addgene plasmid #99231

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    flex-ChrimsonR-EYFP-Kv was a gift from Hongkui Zeng (Addgene plasmid # 135319 ; http://n2t.net/addgene:135319 ; RRID:Addgene_135319)

  • For your References section:

    Synaptic connectivity to L2/3 of primary visual cortex measured by two-photon optogenetic stimulation. Hage TA, Bosma-Moody A, Baker CA, Kratz MB, Campagnola L, Jarsky T, Zeng H, Murphy GJ. Elife. 2022 Jan 21;11:e71103. doi: 10.7554/eLife.71103. 10.7554/eLife.71103 PubMed 35060903
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