pPS1372
(Plasmid
#1353)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1353 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonena
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepADH-NLS-NES-GFP2
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Speciesna
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PCR fragments encoding ADH1 promoter, simian virus 40 (SV40) NLS, protein kinase inhibitor (PKI) NES, and GFP inserted into GFP plasmid pKG50, 2 micron ori. NLS-NES-GFP encodes a 63-kDa fusion protein. See author's map.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPS1372 was a gift from Pamela Silver (Addgene plasmid # 1353 ; http://n2t.net/addgene:1353 ; RRID:Addgene_1353) -
For your References section:
A member of the Ran-binding protein family, Yrb2p, is involved in nuclear protein export. Taura T, Krebber H, Silver PA. Proc Natl Acad Sci U S A 1998 Jun 23;95(13):7427-32. 10.1073/pnas.95.13.7427 PubMed 9636166
Map uploaded by the depositor.
