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pcDNA5-FRT-T0-eGFP-HROB
(Plasmid #135299)

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Item Catalog # Description Quantity Price (USD)
Plasmid 135299 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pcDNA5-FRT-T0
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5834
  • Total vector size (bp) 7775
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HROB
  • Alt name
    C17orf53
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1941
  • GenBank ID
    NM_001171251.3
  • Entrez Gene
    HROB (a.k.a. C17orf53, MCM8IP)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-fwd
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-FRT-T0-eGFP-HROB was a gift from Daniel Durocher (Addgene plasmid # 135299 ; http://n2t.net/addgene:135299 ; RRID:Addgene_135299)

  • For your References section:

    Control of homologous recombination by the HROB-MCM8-MCM9 pathway. Hustedt N, Saito Y, Zimmermann M, Alvarez-Quilon A, Setiaputra D, Adam S, McEwan A, Yuan JY, Olivieri M, Zhao Y, Kanemaki MT, Jurisicova A, Durocher D. Genes Dev. 2019 Oct 1;33(19-20):1397-1415. doi: 10.1101/gad.329508.119. Epub 2019 Aug 29. 10.1101/gad.329508.119 PubMed 31467087
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