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Addgene

BS.YxiN.Twin1.a1-368.wt
(Plasmid #13528)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13528 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTWIN1
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 7375
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    B. subtilis DEAD-box helicase YxiN first two domains
  • Alt name
    DbpA homolog
  • Species
    Bacillus subtilis
  • Insert Size (bp)
    1104
  • Mutation
    deleted amino acid residues 369-479
  • GenBank ID
    D83026
  • Tag / Fusion Protein
    • intein-chitin binding domain (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Olke Uhlenbeck, Northwestern University

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BS.YxiN.Twin1.a1-368.wt was a gift from David McKay (Addgene plasmid # 13528 ; http://n2t.net/addgene:13528 ; RRID:Addgene_13528)

  • For your References section:

    YxiN is a modular protein combining a DEx(D/H) core and a specific RNA-binding domain. Karginov FV, Caruthers JM, Hu Y, McKay DB, Uhlenbeck OC. J Biol Chem. 2005 Oct 21. 280(42):35499-505. 10.1074/jbc.M506815200 PubMed 16118224
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