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Addgene

p35S::mCh-HA-P2A-lifeact-mCh
(Plasmid #135234)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135234 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGGZ001
  • Backbone manufacturer
    Addgene plasmid # 48868
  • Backbone size w/o insert (bp) 2687
  • Total vector size (bp) 8383
  • Modifications to backbone
    N/A
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCh-HA-P2A-lifeact-mCh
  • Insert Size (bp)
    1621
  • Promoter 35S
  • Tags / Fusion Proteins
    • HA (C terminal on insert)
    • p2a (C terminal on insert)
    • lifeact (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer TCAAAGCAAGTGGATTGATG
  • 3′ sequencing primer GAAAGAGATAACAGGAACGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/825190v1?rss=1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p35S::mCh-HA-P2A-lifeact-mCh was a gift from Armin Djamei (Addgene plasmid # 135234 ; http://n2t.net/addgene:135234 ; RRID:Addgene_135234)

  • For your References section:

    A high-throughput screening method to identify proteins involved in unfolded protein response of the endoplasmic reticulum in plants. Alcantara A, Seitner D, Navarrete F, Djamei A. Plant Methods. 2020 Jan 21;16:4. doi: 10.1186/s13007-020-0552-3. eCollection 2020. 10.1186/s13007-020-0552-3 PubMed 31988651
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