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Addgene

pBR322_ZA-ampR-PSEAB-2A
(Plasmid #135230)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135230 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    aac6 Pseudomonas sp. ABAC61
  • Species
    Pseudomonas sp. ABAC61
  • Insert Size (bp)
    447
  • Promoter pZA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (unknown if destroyed)
  • 3′ cloning site BsaI (unknown if destroyed)
  • 5′ sequencing primer atgcttCAATAATATTGACA
  • 3′ sequencing primer atatgagtAAACTTGGTCTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBR322_ZA-ampR-PSEAB-2A was a gift from Chris Sander (Addgene plasmid # 135230 ; http://n2t.net/addgene:135230 ; RRID:Addgene_135230)

  • For your References section:

    Protein Structure from Experimental Evolution. Stiffler MA, Poelwijk FJ, Brock KP, Stein RR, Riesselman A, Teyra J, Sidhu SS, Marks DS, Gauthier NP, Sander C. Cell Syst. 2020 Jan 22;10(1):15-24.e5. doi: 10.1016/j.cels.2019.11.008. Epub 2019 Dec 11. 10.1016/j.cels.2019.11.008 PubMed 31838147
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