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pBR322_KanR_AgeI_AvrII_PSE1
(Plasmid #135229)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135229 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PSE-1
  • Species
    Pseudomonas aeruginosa
  • Insert Size (bp)
    813

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site AvrII (unknown if destroyed)
  • 5′ sequencing primer ATGAGTATTCAACATTTCCGTGTCG
  • 3′ sequencing primer CTTCACCTAGATCCTTTTAAATTAAAAATGAAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBR322_KanR_AgeI_AvrII_PSE1 was a gift from Chris Sander (Addgene plasmid # 135229 ; http://n2t.net/addgene:135229 ; RRID:Addgene_135229)

  • For your References section:

    Protein Structure from Experimental Evolution. Stiffler MA, Poelwijk FJ, Brock KP, Stein RR, Riesselman A, Teyra J, Sidhu SS, Marks DS, Gauthier NP, Sander C. Cell Syst. 2020 Jan 22;10(1):15-24.e5. doi: 10.1016/j.cels.2019.11.008. Epub 2019 Dec 11. 10.1016/j.cels.2019.11.008 PubMed 31838147
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