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GC52 pEGFPN1 anti-Gephyrin nanobody
(Plasmid #135224)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135224 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    anti-Gephyrin (Human) recombinant llama nanobody GC52
  • Species
    L. glama (llama)
  • Entrez Gene
    GPHN (a.k.a. GEPH, GPH, GPHRYN, HKPX1, MOCODC)
  • Tag / Fusion Protein
    • 6xHis-HA-EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GC52 pEGFPN1 anti-Gephyrin nanobody was a gift from James Trimmer (Addgene plasmid # 135224 ; http://n2t.net/addgene:135224 ; RRID:Addgene_135224)

  • For your References section:

    A toolbox of nanobodies developed and validated for use as intrabodies and nanoscale immunolabels in brain neurons. Dong JX, Lee Y, Kirmiz M, Palacio S, Dumitras C, Moreno CM, Sando R, Santana LF, Sudhof TC, Gong B, Murray KD, Trimmer JS. Elife. 2019 Sep 30;8. pii: 48750. doi: 10.7554/eLife.48750. 10.7554/eLife.48750 PubMed 31566565
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