pPS2351
(Plasmid #1352)

• Depositing Lab: Pamela Silver
• Publication: Hitchcock et al Mol Biol Cell 2001 Oct;12(10):3226-41.

Backbone

• Vector backbone: pPS808
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MGA2
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: MGA2 (a.k.a. YIR033W)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: GFP-F
• 3′ sequencing primer: M13pUC-Fwd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Expresses both the unprocessed and processed forms of MGA2. pPS808 (or pCGF-1a) is YEp352-derived vector with GAL1 promoter (EcoRI/XbaI fragment), GFP (BglII/BamHI fragment), and 2 micron ori.