pSNK
(Plasmid
#135193)
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PurposeArabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassette
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 135193 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepKOBEG
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Modifications to backboneKan cassette inserted in place of Cm cassette
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameI-SceI lamba Red
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SpeciesSynthetic
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MutationNone
- Promoter araBAD
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byPlasmid pKOBEG, used for cloning the lamba red system, was a gift from Christophe d'Enfert
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSNK was a gift from Hongqing Fang (Addgene plasmid # 135193 ; http://n2t.net/addgene:135193 ; RRID:Addgene_135193) -
For your References section:
An electroporation-free method based on Red recombineering for markerless deletion and genomic replacement in the Escherichia coli DH1 genome. Wei Y, Deng P, Mohsin A, Yang Y, Zhou H, Guo M, Fang H. PLoS One. 2017 Oct 24;12(10):e0186891. doi: 10.1371/journal.pone.0186891. eCollection 2017. 10.1371/journal.pone.0186891 PubMed 29065183