pCNA
(Plasmid #135191)

• Purpose: Arabinose inducible expression of lamba-Red recombinase proteins and I-CreI.
• Depositing Lab: Hongqing Fang
• Publication: Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. doi: 10.1371/journal.pone.0186891. eCollection 2017.

Backbone

• Vector backbone: pKOBEG
• Modifications to backbone: Amp cassette inserted in place of Cm cassette
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: I-CreI lamba Red
• Species: Synthetic
• Mutation: None
• Promoter: araBAD

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pCNA.gb
• A portion of this plasmid was derived from a plasmid made by: Plasmid pKOBEG, used for cloning the lamba red system, was a gift from Christophe d'Enfert

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry