pBDK
(Plasmid #135190)

• Purpose: Integration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection
• Depositing Lab: Hongqing Fang
• Publication: Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. doi: 10.1371/journal.pone.0186891. eCollection 2017.

Backbone

• Vector backbone: N/A
• Modifications to backbone: SacB selection cassette, p15A Ori
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: KanR
• Species: Synthetic
• Promoter: cat

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13 F or T7 F
• 3′ sequencing primer: M13 R or T7 R

Resource Information

• Supplemental Documents:
  • pBDK.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains two multiple cloning sites for insertion of homologous regions for targeted deletion via lamba red system in E. coli.

Contains I-CreI flanking the Kan cassette outside of the flanking multiple cloning sites for excision of homologous regions and Kan marker from the plasmid.

Contains I-SceI sites flanking the Kan cassette to allow for excision of the Kan cassette after integration into the E. coli genome for markerless deletion.