pCDNA3-Flag MKK6(K82A)
(Plasmid #13519)

• Depositing Lab: Roger Davis
• Publication: Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Backbone

• Vector backbone: pCDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MKK6(K82A)
• Alt name: MKK6
• Species: H. sapiens (human)
• Insert Size (bp): 1235
• Mutation: Lys-82Ala
• Entrez Gene: MAP2K6 (a.k.a. MAPKK6, MEK6, MKK6, PRKMK6, SAPKK-3, SAPKK3)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note there are 3 additional mutations found in the QC sequence that are not present in the NGS full sequencing result. These mutations have no affect on the plasmid function.