pBDC
(Plasmid
#135188)
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PurposeIntegration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 135188 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneN/A
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Modifications to backboneSacB selection cassette, p15A Ori
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCmR
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SpeciesSynthetic
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MutationNone
- Promoter cat
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13 F or T7 F
- 3′ sequencing primer M13 R or T7 R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains two multiple cloning sites for insertion of homologous regions for targeted deletion via lamba red system in E. coli.
Contains I-CreI flanking the Cm cassette outside of the flanking multiple cloning sites for excision of homologous regions and CmR marker from the plasmid.
Contains I-SceI sites flanking the Cm cassette to allow for excision of the Cm cassette after integration into the E. coli genome for markerless deletion.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBDC was a gift from Hongqing Fang (Addgene plasmid # 135188 ; http://n2t.net/addgene:135188 ; RRID:Addgene_135188) -
For your References section:
An electroporation-free method based on Red recombineering for markerless deletion and genomic replacement in the Escherichia coli DH1 genome. Wei Y, Deng P, Mohsin A, Yang Y, Zhou H, Guo M, Fang H. PLoS One. 2017 Oct 24;12(10):e0186891. doi: 10.1371/journal.pone.0186891. eCollection 2017. 10.1371/journal.pone.0186891 PubMed 29065183