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Addgene

pBDC
(Plasmid #135188)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135188 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    N/A
  • Modifications to backbone
    SacB selection cassette, p15A Ori
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CmR
  • Species
    Synthetic
  • Mutation
    None
  • Promoter cat

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains two multiple cloning sites for insertion of homologous regions for targeted deletion via lamba red system in E. coli.

Contains I-CreI flanking the Cm cassette outside of the flanking multiple cloning sites for excision of homologous regions and CmR marker from the plasmid.

Contains I-SceI sites flanking the Cm cassette to allow for excision of the Cm cassette after integration into the E. coli genome for markerless deletion.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBDC was a gift from Hongqing Fang (Addgene plasmid # 135188 ; http://n2t.net/addgene:135188 ; RRID:Addgene_135188)
  • For your References section:

    An electroporation-free method based on Red recombineering for markerless deletion and genomic replacement in the Escherichia coli DH1 genome. Wei Y, Deng P, Mohsin A, Yang Y, Zhou H, Guo M, Fang H. PLoS One. 2017 Oct 24;12(10):e0186891. doi: 10.1371/journal.pone.0186891. eCollection 2017. 10.1371/journal.pone.0186891 PubMed 29065183
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