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PurposeAAV expression of TRE(TRE3G)-driven, tdTomato expression for fluorescent labling
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 135178 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (addgene plasmid #35509)
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Backbone manufactureraddgene plasmid #35509
- Backbone size w/o insert (bp) 4041
- Total vector size (bp) 5887
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Modifications to backbonePromoter and CDS parts were excised from the backbone plasmid by MluI and BamHI. TRE3G promoter and tdTomato were inserted.
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nametdTomato
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SpeciesDiscosoma sp.
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Insert Size (bp)1428
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Mutationa variant of DsRed
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GenBank IDKJ081243.1
- Promoter TRE3G
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer nd (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameTRE3G
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SpeciesSynthetic
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Insert Size (bp)376
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer nd (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byTRE3G promoter was obtained from pTRE3G-ZsGreen1 of Clontech. The use of TRE3G is licensed from TETsystems Inc.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This AAV plasmid has been used in Abe et al., 2018, (PMID 30425625) and Lin et al, 2019, (PMID 30720427).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAVTRE3_tdTomato was a gift from Tetsuo Yamamori (Addgene plasmid # 135178 ; http://n2t.net/addgene:135178 ; RRID:Addgene_135178) -
For your References section:
Axonal Projections From the Middle Temporal Area in the Common Marmoset. Abe H, Tani T, Mashiko H, Kitamura N, Hayami T, Watanabe S, Sakai K, Suzuki W, Mizukami H, Watakabe A, Yamamori T, Ichinohe N. Front Neuroanat. 2018 Oct 30;12:89. doi: 10.3389/fnana.2018.00089. eCollection 2018. 10.3389/fnana.2018.00089 PubMed 30425625