pAAV-TRE-flex-Clover
(Plasmid #135177)

• Purpose: AAV expression of TRE(TRE3G)-driven, Cre-dependent, Clover expression for fluorescent labling
• Depositing Lab: Tetsuo Yamamori
• Publication: Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116(45):22844-22850. doi: 10.1073/pnas.1903445116. Epub 2019 Oct 21.

Backbone

• Vector backbone: pAAV
• Backbone manufacturer: Stratagene
• Modifications to backbone: The promoter is replaced by TRE3 promoter of pTRE3G-ZsGreen1.
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Clover
• Species: Aequorea victoria
• Mutation: a variant of GFP
• Promoter: TRE3G

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: nd

Gene/Insert 2

• Gene/Insert name: TRE3G
• Species: Synthetic

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: nd

Resource Information

• Supplemental Documents:
  • pAAV-TRE3Gpro-DFIO-Clover-WPRE-polyA.ape
• A portion of this plasmid was derived from a plasmid made by: Clover was obtained from addgene plasmid #40259. TRE3G promoter was obtained from pTRE3G-ZsGreen1 of Clontech. The use of TRE3G is licensed from TETsystems Inc.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry