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Addgene

CI-3
(Plasmid #135166)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135166 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pH9
  • Backbone size w/o insert (bp) 3100
  • Total vector size (bp) 4900
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CysJ-infA overlapping region in CI-3
  • Species
    Synthetic
  • Insert Size (bp)
    1800
  • Promoter TetO

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACCTCATTAAGCAGCTCTAATGCGCTG
  • 3′ sequencing primer TGTGACTCTAGTAGAGAGCGTTCAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CI-3 was a gift from Harris Wang (Addgene plasmid # 135166 ; http://n2t.net/addgene:135166 ; RRID:Addgene_135166)

  • For your References section:

    Synthetic sequence entanglement augments stability and containment of genetic information in cells. Blazejewski T, Ho HI, Wang HH. Science. 2019 Aug 9;365(6453):595-598. doi: 10.1126/science.aav5477. 10.1126/science.aav5477 PubMed 31395784
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