pDctp-mSox10-Mp1
(Plasmid #13513)

• Depositing Lab: William Pavan
• Publication: Hakami et al Mech Dev. 2006 Feb . 123(2):124-34.

Backbone

• Vector backbone: pBluescript II SK(+)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Sox10
• Species: M. musculus (mouse)
• Insert Size (bp): 6200
• GenBank ID: AF017182
• Entrez Gene: Sox10 (a.k.a. Dom, Sox21, gt)
• Tags / Fusion Proteins:
  • Dct promoter (N terminal on insert)
  • Mp1-polyA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: EcoRV (unknown if destroyed)
• 5′ sequencing primer: don't know
• 3′ sequencing primer: don't know

Resource Information

• Supplemental Documents:
  • Feature locations in sequence
• A portion of this plasmid was derived from a plasmid made by: pMG1, Dr. Glenn Merlino, NCI, NIH.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Dct promoter sequence in Dr. Merlino's plasmid, published
by Dr. Ian Jackson, 1995