pPS2348
(Plasmid
#1351)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1351 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPS808
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSPT23
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneSPT23
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SphI (not destroyed)
- 3′ cloning site SalI (destroyed during cloning)
- 5′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Expresses both the unprocessed and processed forms of Spt23. pPS808 (or pCGF-1a) is YEp352-derived vector with GAL1 promoter (EcoRI/XbaI fragment), GFP (BglII/BamHI fragment), and 2 micron ori.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPS2348 was a gift from Pamela Silver (Addgene plasmid # 1351 ; http://n2t.net/addgene:1351 ; RRID:Addgene_1351) -
For your References section:
The conserved npl4 protein complex mediates proteasome-dependent membrane-bound transcription factor activation. Hitchcock AL, Krebber H, Frietze S, Lin A, Latterich M, Silver PA. Mol Biol Cell 2001 Oct;12(10):3226-41. 10.1091/mbc.12.10.3226 PubMed 11598205