pcDNA3.1(-)-mIFNa2
(Plasmid #135098)

• Purpose: Protein expression of mouse IFNa2 in mammalian cells
• Depositing Lab: Pablo Penaloza-MacMaster
• Publication: Palacio et al J Exp Med. 2020 Dec 7;217(12). pii: 152035. doi: 10.1084/jem.20191220.

Backbone

• Vector backbone: pcDNA3.1(-)
• Backbone size w/o insert (bp): 5430
• Total vector size (bp): 6022
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mIFN-α2
• Alt name: Ifa2
• Species: M. musculus (mouse)
• Insert Size (bp): 626
• Entrez Gene: Ifna2 (a.k.a. Ifa2)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 primer
• 3′ sequencing primer: BGH Reverse Primer

Resource Information

• Supplemental Documents:
  • pcDNA3.1(-)-mIFNa2.gb
• A portion of this plasmid was derived from a plasmid made by: mIFNa insert cloned from plasmid RDC2321 (R&D Systems)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry