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Addgene

pCZGY847
(Plasmid #135093)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135093 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST
  • Backbone manufacturer
    Invitrogen Gateway
  • Backbone size w/o insert (bp) 4045
  • Total vector size (bp) 5720
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Pacr-2
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1888
  • Promoter Pacr-2
  • Tags / Fusion Proteins
    • mcherry (C terminal on backbone)
    • unc-10 3'UTR (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer AAAAGCAGGCTCCGAATTGG
  • 3′ sequencing primer GGCTGCAGGAATTCGATATCA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCZGY847 was a gift from Yishi Jin (Addgene plasmid # 135093 ; http://n2t.net/addgene:135093 ; RRID:Addgene_135093)

  • For your References section:

    A neuronal acetylcholine receptor regulates the balance of muscle excitation and inhibition in Caenorhabditis elegans. Jospin M, Qi YB, Stawicki TM, Boulin T, Schuske KR, Horvitz HR, Bessereau JL, Jorgensen EM, Jin Y. PLoS Biol. 2009 Dec;7(12):e1000265. doi: 10.1371/journal.pbio.1000265. Epub 2009 Dec 22. 10.1371/journal.pbio.1000265 PubMed 20027209
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