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pET28a-MBP-(CBX1)3-eReader
(Plasmid #135090)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135090 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) -4
  • Total vector size (bp) 7005
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CBX1 chromobox protein 1, engineered x3
  • Alt name
    CBX1 eR
  • Species
    H. sapiens (human)
  • Mutation
    lysine 43 mutated to alanine; aspartic acid 59 mutated to phenylalanine
  • Promoter T7
  • Tags / Fusion Proteins
    • MBP (N terminal on backbone)
    • 6xhis-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-MBP-(CBX1)3-eReader was a gift from Marcey Waters (Addgene plasmid # 135090 ; http://n2t.net/addgene:135090 ; RRID:Addgene_135090)

  • For your References section:

    Engineered Reader Proteins for Enhanced Detection of Methylated Lysine on Histones. Albanese KI, Krone MW, Petell CJ, Parker MM, Strahl BD, Brustad EM, Waters ML. ACS Chem Biol. 2020 Jan 17;15(1):103-111. doi: 10.1021/acschembio.9b00651. Epub 2019 Nov 1. 10.1021/acschembio.9b00651 PubMed 31634430
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