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Addgene

pJeM1
(Plasmid #135088)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135088 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJOE4776.1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    rhaR rhaS rhaPBAD eGFP, mob
  • Species
    E.coli
  • Entrez Gene
    rhaR (a.k.a. b3906, ECK3899, rhaC1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJeM1 was a gift from Josef Altenbuchner (Addgene plasmid # 135088 ; http://n2t.net/addgene:135088 ; RRID:Addgene_135088)

  • For your References section:

    The Escherichia coli rhamnose promoter rhaP(BAD) is in Pseudomonas putida KT2440 independent of Crp-cAMP activation. Jeske M, Altenbuchner J. Appl Microbiol Biotechnol. 2010 Feb;85(6):1923-33. doi: 10.1007/s00253-009-2245-8. Epub 2009 Sep 30. 10.1007/s00253-009-2245-8 PubMed 19789867
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