pJOE3075.3
(Plasmid
#135084)
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PurposeL-rhamnose-inducible expression vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 135084 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneunknown
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameP-rhaP-BAD-malE
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SpeciesOther
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJOE3075.3 was a gift from Josef Altenbuchner (Addgene plasmid # 135084 ; http://n2t.net/addgene:135084 ; RRID:Addgene_135084) -
For your References section:
Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations. Wegerer A, Sun T, Altenbuchner J. BMC Biotechnol. 2008 Jan 14;8:2. doi: 10.1186/1472-6750-8-2. 10.1186/1472-6750-8-2 PubMed 18194555