pJOE6090.1
(Plasmid
#135080)
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PurposepBR322-based L-rhamnose-inducible vector.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 135080 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBR322
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameP-rhaP-BAD-Strep-tag II-eGFP
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SpeciesEscherichia coli rhamnose promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains a S30Y mutation in EGFP. This mutation is not known to affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJOE6090.1 was a gift from Josef Altenbuchner (Addgene plasmid # 135080 ; http://n2t.net/addgene:135080 ; RRID:Addgene_135080) -
For your References section:
Functional Characterization of the Mannitol Promoter of Pseudomonas fluorescens DSM 50106 and Its Application for a Mannitol-Inducible Expression System for Pseudomonas putida KT2440. Hoffmann J, Altenbuchner J. PLoS One. 2015 Jul 24;10(7):e0133248. doi: 10.1371/journal.pone.0133248. eCollection 2015. 10.1371/journal.pone.0133248 PubMed 26207762
