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Addgene

pJOE6261.2
(Plasmid #135079)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135079 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJOE6186.1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    upp
  • Species
    Pseudomonas putida
  • Entrez Gene
    upp (a.k.a. PP_0746)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJOE6261.2 was a gift from Josef Altenbuchner (Addgene plasmid # 135079 ; http://n2t.net/addgene:135079 ; RRID:Addgene_135079)

  • For your References section:

    Development of a method for markerless gene deletion in Pseudomonas putida. Graf N, Altenbuchner J. Appl Environ Microbiol. 2011 Aug;77(15):5549-52. doi: 10.1128/AEM.05055-11. Epub 2011 Jun 10. 10.1128/AEM.05055-11 PubMed 21666018
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