pJOE6227.1
(Plasmid
#135078)
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PurposeAn integration vector for the chromosomal deletion of the upp gene in P. putida KT2440; carries the upp deletion cassette.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 135078 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepJOE6186.1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameup- and downstream regions of upp
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SpeciesPseudomonas putida
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Entrez Geneupp (a.k.a. PP_0746)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJOE6227.1 was a gift from Josef Altenbuchner (Addgene plasmid # 135078 ; http://n2t.net/addgene:135078 ; RRID:Addgene_135078) -
For your References section:
Development of a method for markerless gene deletion in Pseudomonas putida. Graf N, Altenbuchner J. Appl Environ Microbiol. 2011 Aug;77(15):5549-52. doi: 10.1128/AEM.05055-11. Epub 2011 Jun 10. 10.1128/AEM.05055-11 PubMed 21666018
