pJOE7771.1
(Plasmid
#135077)
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PurposeMannitol, arabitol, and glucitol-inducible mtlR/PmtlE expression vector
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 135077 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepJOE4776.1
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Vector typeBacterial Expression
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Selectable markerseGFP
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemtlR
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SpeciesPseudomonas fluorescens A506
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJOE7771.1 was a gift from Josef Altenbuchner (Addgene plasmid # 135077 ; http://n2t.net/addgene:135077 ; RRID:Addgene_135077) -
For your References section:
Functional Characterization of the Mannitol Promoter of Pseudomonas fluorescens DSM 50106 and Its Application for a Mannitol-Inducible Expression System for Pseudomonas putida KT2440. Hoffmann J, Altenbuchner J. PLoS One. 2015 Jul 24;10(7):e0133248. doi: 10.1371/journal.pone.0133248. eCollection 2015. 10.1371/journal.pone.0133248 PubMed 26207762
