pJH181.3
(Plasmid
#135076)
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PurposeCoryne. glutamicum HA production plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 135076 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJOE7706.1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehasA-hasC
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SpeciesStreptococcus equi subsp. Zooepidemicus, Pseudomonas putida KT2440
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJH181.3 was a gift from Josef Altenbuchner (Addgene plasmid # 135076 ; http://n2t.net/addgene:135076 ; RRID:Addgene_135076) -
For your References section:
Hyaluronic acid production with Corynebacterium glutamicum: effect of media composition on yield and molecular weight. Hoffmann J, Altenbuchner J. J Appl Microbiol. 2014 Sep;117(3):663-78. doi: 10.1111/jam.12553. Epub 2014 Jun 20. 10.1111/jam.12553 PubMed 24863652