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Purpose(Empty Backbone) A shuttle vector for heterologous HA production with Corynebacterium glutamicum
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 135075 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJOE7706.1
- Backbone size (bp) 8668
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Vector typeBacterial Expression
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Selectable markerseGFP
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains a S30Y mutation in EGFP. This mutation is not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJOE7706.1 was a gift from Josef Altenbuchner (Addgene plasmid # 135075 ; http://n2t.net/addgene:135075 ; RRID:Addgene_135075) -
For your References section:
Hyaluronic acid production with Corynebacterium glutamicum: effect of media composition on yield and molecular weight. Hoffmann J, Altenbuchner J. J Appl Microbiol. 2014 Sep;117(3):663-78. doi: 10.1111/jam.12553. Epub 2014 Jun 20. 10.1111/jam.12553 PubMed 24863652