pcDNA3 Flag FKHR
(Plasmid #13507)

• Depositing Lab: Kunliang Guan
• Publication: Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-6.

Backbone

• Vector backbone: pcDNA3 Flag
• Backbone manufacturer: made in Guan Lab
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FKHR
• Alt name: FOXO1A
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Entrez Gene: FOXO1 (a.k.a. FKH1, FKHR, FOXO1A)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 28 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

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