pGEX4T1-Smurf1 C699A
(Plasmid #13503)

• Depositing Lab: Jeff Wrana
• Publication: Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Backbone

• Vector backbone: pGEX-4T1
• Backbone size w/o insert (bp): 4900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SMURF1
• Species: H. sapiens (human)
• Insert Size (bp): 2100
• Mutation: C699A: catalytically inactive
• GenBank ID: NM_020429
• Entrez Gene: SMURF1
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: pGEX 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Vector has TEV site.

A human Smurf1 cDNA encoding all but the first eight amino acids was identified in the expressed-sequence-tag database (AA292123), and full-length hSmurf1 was reconstituted using a fragment of XSmurf1 cDNA encoding the first eight amino acids.