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Addgene

N-4-BE
(Plasmid #135019)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135019 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Px330
  • Modifications to backbone
    synthesized UGI-2A-EGFP cassette added to vector
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LjCDA1L1_4
  • Species
    Lamprey
  • Promoter U6 with EF-1alpha intron

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    N-4-BE was a gift from Tian-Lin Cheng & Zilong Qiu (Addgene plasmid # 135019 ; http://n2t.net/addgene:135019 ; RRID:Addgene_135019)

  • For your References section:

    Expanding C-T base editing toolkit with diversified cytidine deaminases. Cheng TL, Li S, Yuan B, Wang X, Zhou W, Qiu Z. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6. 10.1038/s41467-019-11562-6 PubMed 31399578
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