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Addgene

pMP650
(Plasmid #135000)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135000 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW
  • Backbone manufacturer
    David Baltimore Lab Addgene #14883
  • Total vector size (bp) 10743
  • Vector type
    Mammalian Expression, Lentiviral, Synthetic Biology
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoR
  • Species
    Synthetic
  • Mutation
    DpnI truncated to aa146-254
  • Tags / Fusion Proteins
    • DpnI(aa146-254) (N terminal on insert)
    • VP-64
    • V5 tag (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMP650 was a gift from Ahmad Khalil (Addgene plasmid # 135000 ; http://n2t.net/addgene:135000 ; RRID:Addgene_135000)

  • For your References section:

    Engineering Epigenetic Regulation Using Synthetic Read-Write Modules. Park M, Patel N, Keung AJ, Khalil AS. Cell. 2019 Jan 10;176(1-2):227-238.e20. doi: 10.1016/j.cell.2018.11.002. Epub 2018 Dec 6. 10.1016/j.cell.2018.11.002 PubMed 30528434
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